Lysozyme and bovine serum albumin partitioning in polyethylene glycol–phenylalanine conjugate polymer/salt aqueous two-phase systems
نویسندگان
چکیده
منابع مشابه
Characterization of bovine serum albumin partitioning behaviors in polymer-salt aqueous two-phase systems.
In this paper, a linear relationship is proposed relating the natural logarithm of partition coefficient, ln K for protein partitioning in poly (ethylene glycol) (PEG)-phosphate aqueous two-phase system (ATPS) to the square of tie-line length (TLL(2)). This relationship provides good fits (r(2) > 0.98) to the partition of bovine serum albumin (BSA) in PEG (1450 g/mol, 2000 g/mol, 3350 g/mol, an...
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A relationship is proposed for the interfacial partitioning of protein in poly(ethylene glycol) (PEG)-phosphate aqueous two-phase system (ATPS). The relationship relates the natural logarithm of interfacial partition coefficient, ln G to the PEG concentration difference between the top and bottom phases, Δ[PEG], with the equation ln G=AΔ[PEG]+B. Results showed that this relationship provides go...
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The partitioning of lysozyme and extraction yield in an aqueous two-phase system containing Poly Vinyl Pyrrolidone (PVP) K25 and potassium phosphate were investigated as a function of weight percent of salt and PVP in the feed, temperature, and pH. To investigate partitioning behavior, the central composite design was considered using a quadratic model. According to the results of the model...
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The objective of this study was to determine the thermodynamic parameters (Delta(tr)G, Delta(tr)H and Delta(tr)S) associated with lysozyme and conalbumin partitioning in aqueous two-phases systems (ATPS). Influence of salt type and polyethylene glycol (PEG) concentrations on the partition coefficient of lysozyme and conalbumin from egg white was studied. The evaluated ATPS were composed of PEG ...
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for use in extracorporeal circuits, for the removal of toxic substances such as certain metabolites or antibodies from blood. For example, exogenous human serum amyloid P component, a substance associated with Alzheimer’s disease, has been removed from whole rat blood in an extracorporeal circulation system. This model system used a polyclonal antibody coupled to cellulose Sat-sheet membranes. ...
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ژورنال
عنوان ژورنال: Fluid Phase Equilibria
سال: 2012
ISSN: 0378-3812
DOI: 10.1016/j.fluid.2012.03.003